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Targeted Allele Detail
Symbol: Gcsamtm1.1Rsky
Name: germinal center associated, signaling and motility; targeted mutation 1.1, Klaus Rajewsky
MGI ID: MGI:3763735
Synonyms: M17-
Gene: Gcsam  Location: Chr16:45610440-45622867 bp, + strand  Genetic Position: Chr16, 29.72 cM, cytoband B5
Germline Transmission:  Earliest citation of germline transmission: J:125784
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA 4.5 kb region containing exons 4 and 5 of the gene were flanked by loxP sites. A loxP flanked neo cassette was place upstream of exon 4, and a frt-flanked reporter cassette consisting of a splice acceptor site, an IRES domain, and a GFP gene was placed down stream of exon 5. Chimeric mice were crossed with a Cre-expressing transgenic, removing the neo cassette and the two coding exons. Inactivation of the gene was confirmed by RT-PCR on RNA from germinal center B-cells. No GFP expression was detected, possibly due to unstable RNA. (J:125784)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gcsam Mutation:  6 strains or lines available
Original:  J:125784 Schenten D, et al., M17, a gene specific for germinal center (GC) B cells and a prognostic marker for GC B-cell lymphomas, is dispensable for the GC reaction in mice. Blood. 2006 Jun 15;107(12):4849-56
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
The Jackson Laboratory