Ramp1^tm1.1Tsuj
Targeted Allele Detail

Summary

• Symbol: Ramp1^tm1.1Tsuj
• Name: receptor (calcitonin) activity modifying protein 1; targeted mutation 1, Kazutake Tsujikawa
• MGI ID: MGI:3761144
• Gene: Ramp1 Location: Chr1:91107544-91152918 bp, + strand Genetic Position: Chr1, 46.08 cM
• Alliance: Ramp1^tm1.1Tsuj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125974
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated by breeding mice heterozygous for Ramp1^tm1Tsuj with transgenic mice ubiquitously expressing cre recombinase. Exon 2 of the gene and the neomycin resistance cassette were removed, leaving behind a single loxP site. Upon deletion of exon 2, the splicing of exons 1 to 3 created a frameshift that resulted in the introduction of a termination codon at the beginning of exon 3. RT-PCR analysis of the thymus indicated there was no transcription of the wild-type gene. (J:125974)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ramp1 Mutation: 20 strains or lines available

References

• Original: J:125974 Tsujikawa K, et al., Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice. Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16702-7
• All: 20 reference(s)