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Targeted Allele Detail
Symbol: Cd8atm1Asin
Name: CD8 antigen, alpha chain; targeted mutation 1, Alfred Singer
MGI ID: MGI:3760403
Synonyms: CD8.4
Gene: Cd8a  Location: Chr6:71350411-71356155 bp, + strand  Genetic Position: Chr6, 32.14 cM, cytoband C
Germline Transmission:  Earliest citation of germline transmission: J:126026
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Inserted expressed sequence)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA targeting construct was created that includes exons 1-3 that encode the extracellular domain, a floxed neomycin cassette, a chimeric exon 4 encoding the endogenous transmembrane domain ligated to the CD4 cytoplasmic tail, and a truncated version of the noncoding exon 5. The amino acid sequence at the site of gene fusion is IITLICYHR-CRHQQ with the hyphen representing site of gene fusion. Chimeric mice were mated to Cre expressing transgenic mice to induce germline excision of the neomycin-resistance cassette. Expression of the fusion protein was confirmed by immunoprecipitation with anti-CD8 antibodies. (J:126026)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd8a Mutation:  25 strains or lines available
Original:  J:126026 Erman B, et al., Coreceptor signal strength regulates positive selection but does not determine CD4/CD8 lineage choice in a physiologic in vivo model. J Immunol. 2006 Nov 15;177(10):6613-25
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory