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Targeted Allele Detail
Symbol: Rapgef2tm1.1Tkat
Name: Rap guanine nucleotide exchange factor (GEF) 2; targeted mutation 1.1, Tohru Kataoka
MGI ID: MGI:3760243
Synonyms: RA-GEF-1-
Gene: Rapgef2  Location: Chr3:79062516-79286517 bp, - strand  Genetic Position: Chr3, 34.91 cM
Germline Transmission:  Earliest citation of germline transmission: J:125199
Parent Cell Line:  EB3 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsExon 15 was removed by cre-mediated recombination following a cross with Tg(ACTB-cre)13Miya mice. Removal of the exon was confirmed by PCR and absence of a protein product was confirmed by western blot analysis on proteins extracted from E10.5 embryos. (J:125199)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 8 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rapgef2 Mutation:  43 strains or lines available
ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:125199 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.
Original:  J:125199 Wei P, et al., Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1. Biochem Biophys Res Commun. 2007 Nov 9;363(1):106-12
All:  2 reference(s)

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MGI 6.11
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