Phactr4^{Gt(RRA075)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Phactr4^{Gt(RRA075)Byg}
• Name: phosphatase and actin regulator 4; gene trap RRA075, BayGenomics
• MGI ID: MGI:3720739
• Gene: Phactr4 Location: Chr4:132083233-132149759 bp, - strand Genetic Position: Chr4, 65.24 cM
• Alliance: Phactr4^{Gt(RRA075)Byg} page

Mutation origin

• Mutant Cell Line: RRA075
• Germline Transmission: Earliest citation of germline transmission: J:123924
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted into the second intron, resulting in the in-frame fusion of the first five amino acids with a beta-galactosidase and neomycin phosphotransferase cassette. (J:123924)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRA075	CC183566 ; (MGI Seq Detail)	5' RACE	Chr4:132140381-132149763 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  35 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phactr4 Mutation: 61 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:123924 Kim TH, et al., Phactr4 regulates neural tube and optic fissure closure by controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression. Dev Cell. 2007 Jul;13(1):87-102
• All: 2 reference(s)