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Targeted Allele Detail
Symbol: Myctm39Mnz
Name: myelocytomatosis oncogene; targeted mutation 39, Michel C Nussenzweig
MGI ID: MGI:3720515
Synonyms: Mycdelta
Gene: Myc  Location: Chr15:61985391-61990374 bp, + strand  Genetic Position: Chr15, 26.19 cM, cytoband D2-D3
Germline Transmission:  Earliest citation of germline transmission: J:145691
Parent Cell Line:  CY2.4 (ES Cell)
Strain of Origin:  B6(Cg)-Tyrc-2J
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA targeting vector containing a self-excising loxP-flanked neomycin/sperm-specific Cre cassette was used to delete the promoter, first non-coding exon and a portion of intron 1 of the Mycn gene. This 2.2 Kb deletion removed promoters PO, P1, and P2 while leaving promoter P3 intact. The construct was electroporated into CY2.4 albino C57BL/6J-Tyrc-2J-derived embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6J-Tyrc-2J mice to allow for coat-color screening of germline transmission and for self-excision of the neomycin/Cre cassette. RNA-FISH analysis of heterozygote B cells indicated RNA transcription occcured only at one allele compared to the biallelic transcription observed in wild-type B cells. (J:145691)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myc Mutation:  29 strains or lines available
Original:  J:145691 Robbiani DF, et al., AID is required for the chromosomal breaks in c-myc that lead to c-myc/IgH translocations. Cell. 2008 Dec 12;135(6):1028-38
All:  1 reference(s)

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