Dicer1^{Gt(RRF266)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Dicer1^{Gt(RRF266)Byg}
• Name: dicer 1, ribonuclease type III; gene trap RRF266, BayGenomics
• MGI ID: MGI:3719397
• Synonyms: Dicer1^d
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^{Gt(RRF266)Byg} page

Mutation origin

• Mutant Cell Line: RRF266
• Germline Transmission: Earliest citation of germline transmission: J:108137
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted between exons 22 and 23, disrupting the second RNase III domain and resulting in the loss of the double-stranded RNA binding domain. Slight detection of the transcript was detected, indicating leaky expression of the locus caused by an imperfect trap. (J:108137, J:123635)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRF266	CC249122 ; (MGI Seq Detail)	5' RACE	Chr12:104660738-104662810 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  10 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 94 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:108137 Fukasawa M, et al., Genomic imprinting in Dicer1-hypomorphic mice. Cytogenet Genome Res. 2006;113(1-4):138-43
• All: 8 reference(s)