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Transgene Detail
Symbol: Tg(Gfap-TGFB1)64Lms
Name: transgene insertion 64, Lennart Mucke
MGI ID: MGI:3717676
Synonyms: GFAP/TGF-B1, pTGF-B1
Transgene: Tg(Gfap-TGFB1)64Lms  Location: unknown  
Strain of Origin:  (BALB/c x SJL)F2
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Gfap-TGFB1)64Lms expresses 1 gene
Mutation detailsThe construct contains a 1.35 kb porcine transforming growth factor beta 1 (TGFB1) cDNA inserted into the first exon of the mouse glial fibrillary acidic protein (Gfap) gene, making a fusion gene The TGFB1 cDNA has been mutated to encode serines at positions 223 and 225, instead of cysteines, to ensure secretion of a bioactive peptide. The linear construct was microinjected into (BALB/c x SJL)F2 one-cell stage embryos. Line 64 founder mice expressed low levels of TGFB1 mRNA in the brain. Line 115 was also generated and shows very similar properties. (J:27781)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Homozygous trangenic mice develop severe hydrocephalus within 7-20 days after birth and exhibit dwarfism relative to non-transgenic littermates. Mice develop seizures, tremors, incoordination, and hunched postures. Only 1% of heterozygotes developed hydrocephalus.
Original:  J:27781 Wyss-Coray T, et al., Increased central nervous system production of extracellular matrix components and development of hydrocephalus in transgenic mice overexpressing transforming growth factor-beta 1. Am J Pathol. 1995 Jul;147(1):53-67
All:  16 reference(s)

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MGI 6.11
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