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Tg(Col2a1-PTHLH)2Ecw
Transgene Detail
Nomenclature
Symbol: Tg(Col2a1-PTHLH)2Ecw
Name: transgene insertion 2, Eleanor C Weir
MGI ID: MGI:3717404
Synonyms: col II-PTHrP
Transgene: Tg(Col2a1-PTHLH)2Ecw  Location: unknown  
Skeletal phenotypes of wild type, Tg(Col2a1-PTHLH)2Ecw/0, Pthlhtm1Hmk/Pthlhtm1Hmk, and Pthlhtm1Hmk/Pthlhtm1Hmk Tg(Col2a1-PTHLH)2Ecw/0 mice

Show the 6 phenotype image(s) involving this allele.

Transgene
origin
Strain of Origin:  (C57BL/6 x SJL)F2
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsA 568 bp fragment of human PTHLH cDNA under the control of the mouse Col2a1 promoter was used as the transgene. The Col2a1 promoter sequences were used to target expressioin of the transgene to proliferating and prehypertrophic chondrocytes. An artificial exon was engineered into the 5' end of the Col2a1 construct to prevent alternative splicing and the translation initiation codon was inactivated to ensure translation of the human PTHLH gene. 2.2 kb of human growth hormone untranslated sequences were added downstream of PTHLH to provide termination and polyadenylation signals as well as increase expression efficiency. This line exhibits lower expression levels than line 1. (J:76983)
Phenotypes
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:50144 Philbrick WM, et al., Parathyroid hormone-related protein is required for tooth eruption. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11846-51
All:  2 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory