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Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Liqun Luo
MGI ID: MGI:3716464
Synonyms: Gt(ROSA26)ACTB-tdTomato-EGFP, mTmG, mT/mG, mTomato/mEGFP, R26-mR/mG, R26TG, R26-X, Rosa26flox-mTRed-Stop-flox-mGFP, Rosa26mTmG, ROSA26-mTmG, Rosa26mTmGflox, Rosa26RmTmG, ROSAmT-mE, RosamTmG, ROSA-mT/mG, ROSA-R/G, TomatoGFP+
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:124702
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation detailsThe targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, optimized DsRed fluorescent protein variant (called tandem-dimer-Tomato or tdTomato) sequence followed by a polyadenylation signal. Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An frt-flanked neo cassette was also located distal to the expression vector. Red fluorescence is detected in all tissues tested. When mice are bred to Cre expressing mice, the floxed region is excised in Cre-expressing tissue and this allows expression of the EGFP cassette. (J:123053, J:124702)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 270 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  538 strains or lines available
References
Original:  J:124702 Muzumdar MD, et al., A global double-fluorescent Cre reporter mouse. Genesis. 2007 Sep;45(9):593-605
All:  1159 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/15/2020
MGI 6.15
The Jackson Laboratory