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Tg(AMELX*P70T)2Gibs
Transgene Detail
Nomenclature
Symbol: Tg(AMELX*P70T)2Gibs
Name: transgene insertion 2, Carolyn Gibson
MGI ID: MGI:3716166
Transgene: Tg(AMELX*P70T)2Gibs  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6 x SJL
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation details5.5 kb of upstream bovine AMELX gene sequence plus untranslated exon 1, intron 1 and part of exon 2 was fused to a partial mouse Amelx cDNA that encodes the M180 isoform from exon 2 to part of exon 5 and a partial bovine AMELX CDNA that encodes the rest of exon 5 and exon 6, and was followed by a bovine polyadenylation signal sequence from the bovine AMELX gene. Site-directed mutagenesis was used to introduce a mutation in exon 6 that results in a proline to threonine substitution at amino acid 70 of the encoded protein. RT-PCR analysis demonstrated that this transgene is expressed in first mandibular molar teeth in 3-5 day old pups. Western blot analysis demonstrated that transgenic protein was expressed in first mandibular molars. Three lines of mice were produced; in addition to line 2, lines 16 and 29 were also created. (J:122914)
Phenotypes
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Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:122914 Gibson CW, et al., Transgenic mice that express normal and mutated amelogenins. J Dent Res. 2007 Apr;86(4):331-5
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory