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Gfra1tm2Jmi
Targeted Allele Detail
Nomenclature
Symbol: Gfra1tm2Jmi
Name: glial cell line derived neurotrophic factor family receptor alpha 1; targeted mutation 2, Jeffrey Milbrandt
MGI ID: MGI:3715156
Synonyms: Gfra1flox, Gfralpha1floxEGFP
Gene: Gfra1  Location: Chr19:58235604-58455909 bp, - strand  Genetic Position: Chr19, 54.18 cM, cytoband D2-D3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:122607
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA loxP site flanked Gfra1 cDNA (with SV40 poly(A) signal sequences), a GFP gene (with poly(A) signal sequences), and an FRT site flanked neomycin resistance gene cassette were introduced into exon 2. 95 Nucleotides containing the 5' UTR, the initiator ATG codon and the signal sequences, were deleted. Expression of the endogenous gene was abolished, however, the inserted cDNA (but not the GFP gene) was expressed. The neo cassette was removed through subsequent flp-mediated recombination. (J:122607)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 7 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gfra1 Mutation:  5 strains or lines available
References
Original:  J:122607 Uesaka T, et al., Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. Development. 2007 Jun;134(11):2171-81
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/15/2019
MGI 6.14
The Jackson Laboratory