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Targeted Allele Detail
Symbol: Nkd1tm1Kwha
Name: naked cuticle 1; targeted mutation 1, Keith Wharton
MGI ID: MGI:3714460
Synonyms: nkd1lacZ
Gene: Nkd1  Location: Chr8:88521354-88594884 bp, + strand  Genetic Position: Chr8, 43.36 cM, cytoband C4
Germline Transmission:  Earliest citation of germline transmission: J:122353
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129/SvEv
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Northern blot analysis of lung tissue and Western blot analysis of embronic tissue in homozygotes confirm lack of normal mRNA expression, while X-gal staining closely mimics dynamic expression pattern of normal Nkd1 mRNA. (J:122353)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nkd1 Mutation:  4 strains or lines available
Original:  J:122353 Zhang S, et al., Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12):4454-64
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
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