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Targeted Allele Detail
Symbol: Ightm1Mnz
Name: immunoglobulin heavy chain complex; targeted mutation 1, Michel C Nussenzweig
MGI ID: MGI:3712224
Synonyms: B1-8hi
Gene: Igh  Location: unknown  Genetic Position: Chr12, Syntenic
Germline Transmission:  Earliest citation of germline transmission: J:75653
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted
Mutation:    Insertion
Mutation detailsPCR was used to introduce a point mutation conferring higher affinity 4-hydroxy-3-nitrophenyl)acetyl (NP)-binding to Igh-V (VHB1-8): TGG->TTG (codon 33 Trp->Leu). A targeting vector incorporating a 5' floxed neomycin cassette in addition to the point mutation was transfected into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. (J:75653)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Igh Mutation:  37 strains or lines available
Original:  J:75653 Shih TA, et al., Role of antigen receptor affinity in T cell-independent antibody responses in vivo. Nat Immunol. 2002 Apr;3(4):399-406
All:  44 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
The Jackson Laboratory