About   Help   FAQ
Braftm1Mmcm
Targeted Allele Detail
Nomenclature
Symbol: Braftm1Mmcm
Name: Braf transforming gene; targeted mutation 1, Martin McMahon
MGI ID: MGI:3711771
Synonyms: BrafCA, BRAFF-V600E, BRAFV600Eca
Gene: Braf  Location: Chr6:39603237-39725463 bp, - strand  Genetic Position: Chr6, 18.43 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:121715
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to insert a loxP site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a floxed neo cassette. A modified exon 15 was engineered into the vector to encode Braf and a silent XbaI restriction site. RT-PCR confirmed that mutant transcript was expressed at the same level as wild-type, and that the V600E mutation was not detected. (J:121715)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Loading...
Expression
In Structures Affected by this Mutation: 11 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Braf Mutation:  13 strains or lines available
References
Original:  J:121715 Dankort D, et al., A new mouse model to explore the initiation, progression, and therapy of BRAFV600E-induced lung tumors. Genes Dev. 2007 Feb 15;21(4):379-84
All:  97 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
09/10/2019
MGI 6.14
The Jackson Laboratory