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Targeted Allele Detail
Symbol: Col1a1tm2(tetO-Pou5f1)Jae
Name: collagen, type I, alpha 1; targeted mutation 2, Rudolf Jaenisch
MGI ID: MGI:3702325
Synonyms: Col1a1::TetOP-Oct4, TetON-Oct4
Gene: Col1a1  Location: Chr11:94936224-94953042 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Germline Transmission:  Earliest citation of germline transmission: J:98920
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
Mutation:    Insertion
Col1a1tm2(tetO-Pou5f1)Jae expresses 1 gene
Mutation detailsES cells containing the Gt(ROSA)26Sortm1(rtTA2S-M2)Jae mutation were retargeted. Cells were injected with a "flip-in plasmid" containing a tetracycline responsive element (TRE or tetO) and mouse Oct4 (Pou5f1) cDNA sequence (and a Flpe-recombinase plasmid to facilitate tetO-Oct4 integration into the 3' UTR region of Col1a1). With doxycycline treatment, Pou5f1 expression from the Col1a1 locus is widespread with high levels in skin, intestine and stomach, while lower levels are seen in the heart, spleen, thymus and several other organs. No expression is seen in the brain or testes. (J:98920)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  107 strains or lines available
Original:  J:98920 Hochedlinger K, et al., Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005 May 6;121(3):465-77
All:  7 reference(s)

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MGI 6.12
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