Prokr2^tm1Brd
Targeted Allele Detail

Summary

• Symbol: Prokr2^tm1Brd
• Name: prokineticin receptor 2; targeted mutation 1, Allan Bradley
• MGI ID: MGI:3701804
• Synonyms: PKR2^null, Prokr2^Brdm1, Prokr2^m
• Gene: Prokr2 Location: Chr2:132211625-132227413 bp, - strand Genetic Position: Chr2, 64.15 cM
• Alliance: Prokr2^tm1Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119071
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The splice acceptor of exon 3 was duplicated along with 147 bp of coding region and fused to a Hprt containing vector creating a null allele. Targeted mutagenesis was confirmed by RT-PCR for the presence of exons 1, 2 and 3 but the absence of an expressed RNA segment between exons 2 and 3. Radiolabeled ligand (mamba intestinal toxin 1) was also used to confirm the absence of functional protein. (J:119071)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prokr2 Mutation: 42 strains or lines available

References

• Original: J:119071 Prosser HM, et al., Prokineticin receptor 2 (Prokr2) is essential for the regulation of circadian behavior by the suprachiasmatic nuclei. Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):648-53
• All: 4 reference(s)