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QTL Variant Detail
QTL variant: Mlca5C57BL/6J
Name: modifier of locomotor activity 5; C57BL/6J
MGI ID: MGI:3701694
QTL: Mlca5  Location: unknown  Genetic Position: Chr18, Syntenic
Strain of Specimen:  C57BL/6J
Allele Type:    QTL
Inheritance:    Semidominant

Mapping and Phenotype information for this QTL, its variants and associated markers


Genetic loci modifying dopaminergic homeostasis in C57BL/6J-Slc6a3tm1Mca mice were mapped using crosses with ENU-mutagenized DBA/2J males. G1 mutagenized DBA/2J mice were crossed to C57BL/6J-Slc6a3tm1Mca. Progeny derived from three G2 founder mice with increased locomotor phenotype were used for linkage analysis. Genome scan was performed using 99-117 microsatellite markers. Parental strain C57BL/6J-Slc6a3tm1Mca displays significantly increased spontaneous locomotor activity compared to wild type C57BL/6J. Animals were tested by monitoring locomotor activity over a period of 2 hours.

Linkage to locomotor activity was detected at 38.3 cM on mouse Chromosome 2 (LOD=3.7). The broad QTL interval (30 cM - 80 cM) suggests more than one QTL may be present. This locus is designated Mlca1 (modifier of locomotor activity 1). A mutant DBA/2J-derived allele at Mlca1 appears to confer increased spontaneous locomotor activity with semidominant inheritance and the effect is independent of Slc6a3tm1Mca.

Linkage to increased locomotor activity was also detected and confirmed at 67.5 cM on mouse Chromosome 4 (LOD=3.5). This locus is designated Mlca2 (modifier of locomotor activity 2). A mutant DBA/3J-derived allele at Mlca2 appears to enhance locomotor activity with semidominant inheritance and is independent of Slc6a3tm1Mca status.

A major semidominant locus was detected at 49.5 cM on mouse Chromosome 12 (LOD=7.2) in 2 of the founder lines. This locus is designated Mlca3 (modifier of locomotor activity 3). The QTL interval spans approximately 40 cM - 60 cM. A mutant DBA/2J-derived allele at Msla3 appears to confer increased locomotor activity with semidominant inheritance. The effect does not dependent on the presence of the Slc6a3tm1Mca mutation.

Increased locomotor activity in Slc6a3tm1Mca heterozygous progeny mapped to 36.8 cM on mouse Chromosome 16 (LOD=2.9). This locus is designated Mlca4 (modifier of locomotor activity 4). C57BL/6J-derived alleles at Mlca4 enhance locomotor activity in Slc6a3tm1Mca heterozygous animals.

On mouse Chromosome 18, a locus at 33.5 cM exhibits linkage to locomotor activity in Slc6a3tm1Mca heterozygous progeny (LOD=2.5). This locus is designated Mlca5 (modifier of locomotor activity 5). A mutant DBA/2J allele at Mlca5 appears to enhance locomotor activity in Slc6a3tm1Mca heterozygous animals.

Original:  J:118059 Speca DJ, et al., A genetic screen for behavioral mutations that perturb dopaminergic homeostasis in mice. Genes Brain Behav. 2006 Feb;5(1):19-28
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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