Fcs3^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Fcs3^C57BL/6J
• Name: fibroblast cell senescence 3; C57BL/6J
• MGI ID: MGI:3691677
• QTL: Fcs3 Location: unknown Genetic Position: Chr4, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL

Notes

Fcs3 and Fcs4 are an interacting locus pair. Animals homozygous for Pohnpei-derived alleles at both Fcs3 and Fcs4 exhibit the lowest tail skin fibroblast large cell ratios (LCRs). Homozygosity for Pohnpei-derived alleles at Fcs3 and heterozygous at Fcs4, and vice versa, confer increased LCRs.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:114559

Linkage analysis was performed on 162 (Pohnpei x C57BL/6J)F1 x Pohnpei backcross animals to identify loci associated with skin fibroblast senescence. Tail skin fibroblasts (TSFs) from wild-derived parental strain Pohnpei exhibit higher large clone ratios(LCRs) and greater resistance to oxidative stress and cellular senescence-like changes compared to tail skin fibroblasts from parental strain C57BL/6J. 131 SNPs were tested in each mouse.

Fcs1 (fibroblast cell senescence 1) mapped to 55 cM on mouse Chromosome 3 with LOD=2.9. This locus explains 5.3% of the variance. Homozygosity for Pohnpei-derived alleles at Fcs1 confers increased LCRs. Gstm4, Gstm5, and Gstm7 are potential candidate genes for Fcs1.

Fsc2 (fibroblast cell senescence 2) mapped to 50 cM on mouse Chromosome X with LOD=2.6. This locus explains 5.1% of the variance and is influenced by gender. Female animals homozygous for Pohnpei-derived alleles at Fcs2 exhibit significantly lower LCRs.

An interacting locus pair was detected at 51 cM on mouse Chromosome 4 (Fcs3) and 25 cM on mouse Chromosome 10 (Fcs4) with LOD=3.8. This interaction accounts for 8.4% of the variance. Animals homozygous for Pohnpei-derived alleles at both Fcs3 and Fcs4 exhibit the lowest LCRs. Homozygosity for Pohnpei-derived alleles at Fcs3 and heterozygous at Fcs4, and vice versa, confer increased LCRs.

References

• Original: J:114559 Yuan R, et al., Altered growth characteristics of skin fibroblasts from wild-derived mice, and genetic loci regulating fibroblast clone size. Aging Cell. 2006 Jun;5(3):203-12
• All: 1 reference(s)