Slc6a3^tm1(cre)Xz
Targeted Allele Detail

Summary

• Symbol: Slc6a3^tm1(cre)Xz
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 1, Xiaoxi Zhuang
• MGI ID: MGI:3691571
• Synonyms: DAT-cre, Slc6a3^Cre
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^tm1(cre)Xz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115426
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc6a3^tm1(cre)Xz expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc6a3 (MGI:94862)

• Mutation details: A targeting vector was constructed by inserting a cassette with the cre recombinase coding sequence, a nuclear translocation signal, and a PGK-neo gene flanked by FRT sites, into the 5'-UTR of the Slc6a3 gene. After homologous recombination in ES cells, mice with cre recombinase expressed from the Slc6a3 gene were produced. Knockin mice were crossed with transgenic Flp deleter mice to remove the neo marker. A line was established where the neo was removed and the Flp transgene was absent. Cre recombinase expression is detected in the ventral tegmental area (VAT) and substantia nigra zona compacta (SNc). (J:115426)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc6a3 (mouse) Summary of all recombinase alleles driven by Slc6a3. Comparative matrix view of recombinase activities Alliance mouse Slc6a3 gene page

Disease models

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

References

• Original: J:115426 Zhuang X, et al., Targeted gene expression in dopamine and serotonin neurons of the mouse brain. J Neurosci Methods. 2005 Apr 15;143(1):27-32
• All: 90 reference(s)