Chattm1(cre)Lowl
Targeted Allele Detail
|
Symbol: |
Chattm1(cre)Lowl |
Name: |
choline acetyltransferase; targeted mutation 1, Bradford B Lowell |
MGI ID: |
MGI:3689420 |
Synonyms: |
ChAT-IRES-Cre |
Gene: |
Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
|
Alliance: |
Chattm1(cre)Lowl page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:114556
|
Parent Cell Line: |
W4 (ES Cell)
|
Strain of Origin: |
129S6/SvEvTac
|
|
Allele Type: |
|
Targeted (Recombinase) |
Mutation: |
|
Insertion
|
|
|
Chattm1(cre)Lowl expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Chat (MGI:88392) |
|
|
|
|
Mutation details: An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele generated from Chattm2(cre)Lowl.
(J:114556, J:169562)
|
|
|
Activity: |
Tissue activity of this recombinase allele
|
Driver:
|
Chat
(mouse)
|
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
Original: |
J:114556 Lowell BB, et al., Development and phenotype of ChAT-IRES-Cre mice. MGI Direct Data Submission. 2006; |
All: |
122 reference(s) |
|