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Targeted Allele Detail
Symbol: Chattm1(cre)Lowl
Name: choline acetyltransferase; targeted mutation 1, Bradford B Lowell
MGI ID: MGI:3689420
Synonyms: ChAT-IRES-Cre
Gene: Chat  Location: Chr14:32130160-32187866 bp, - strand  Genetic Position: Chr14, 19.4 cM
Germline Transmission:  Earliest citation of germline transmission: J:114556
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
Mutation detailsAn optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele generated from Chattm2(cre)Lowl. (J:114556, J:169562)
 Tissue activity of this recombinase allele
Driver: Chat     Summary of all recombinase alleles driven by Chat. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 21 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chat Mutation:  49 strains or lines available
Original:  J:114556 Lowell BB, et al., Development and phenotype of ChAT-IRES-Cre mice. MGI Direct Data Submission. 2006;
All:  96 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory