Crk^tm1.1Cur
Targeted Allele Detail

Summary

• Symbol: Crk^tm1.1Cur
• Name: v-crk avian sarcoma virus CT10 oncogene homolog; targeted mutation 1.1, Tom Curran
• MGI ID: MGI:3665145
• Gene: Crk Location: Chr11:75570085-75597734 bp, + strand Genetic Position: Chr11, 45.92 cM, cytoband A-D
• Alliance: Crk^tm1.1Cur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111399
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site was inserted 5' to exon 1, and a loxP-flanked neomycin selection cassete was inserted 3' to exon 1. Upon Cre-mediated recombination in ES cells, exon 1 and flanking intronic DNA were deleted, leaving a single loxP site in place of the deleted sequences. Western blot analysis of total protein lysates from MEFs showed that the endogenous CrkI and CrkII proteins were absent in homozyous mice. (J:111399)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crk Mutation: 22 strains or lines available

References

• Original: J:111399 Park TJ, et al., Cardiovascular and craniofacial defects in Crk-null mice. Mol Cell Biol. 2006 Aug;26(16):6272-82
• All: 2 reference(s)