Insm1^tm1Cbm
Targeted Allele Detail

Summary

• Symbol: Insm1^tm1Cbm
• Name: insulinoma-associated 1; targeted mutation 1, Carmen Birchmeier
• MGI ID: MGI:3656035
• Synonyms: Insm1^lacZ
• Gene: Insm1 Location: Chr2:146064021-146066940 bp, + strand Genetic Position: Chr2, 71.33 cM
• Alliance: Insm1^tm1Cbm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112173
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An NLS-lacZ gene followed by a self-excising protamine-cre neomycin selection cassette was fused in-frame to the initiation codon of the gene. Following passage through the male germline of the founder chimeric mouse, the neomycin selection cassette was deleted, leaving the lacZ gene and a single loxP site in place. Beta-galactosidase is expressed from this allele under the control of the endogenous promoter. (J:112173)

Expression

In Mice Carrying this Mutation:	65 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Insm1 Mutation: 13 strains or lines available

References

• Original: J:112173 Gierl MS, et al., The Zinc-finger factor Insm1 (IA-1) is essential for the development of pancreatic beta cells and intestinal endocrine cells. Genes Dev. 2006 Sep 1;20(17):2465-78
• All: 8 reference(s)