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Zfhx2/Zfhx2ostm2Ymri
Targeted Allele Detail
Nomenclature
Symbol: Zfhx2/Zfhx2ostm2Ymri
Name: zinc finger homeobox 2; targeted mutation 2, Tetsuo Yamamori
MGI ID: MGI:3626365
Synonyms: Zfh-5EGFP, Zfhx2/Zfhx2astm2Ymri
Gene: Zfhx2  Location: Chr14:55060262-55092324 bp, - strand  Genetic Position: Chr14, 28.05 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:106421
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsA targeting vector was designed to replace exons 2-6 of Zfhx2 with that of EGFP-pA-neo, such that no functional Zfhx2 mRNA would be produced, but EGFP would be directed by the Zfhx2 promoter. Neither sense (Zfhx2) nor antisense (Zfhx2os) transcripts were expressed except for antisense exons 7-10. In situ hybridization detected ectopic expression of EGFP in the cerebral cortex and pontine nuclei, unlike wild-type expression of Zfhx2. Due to an incomplete termination at the polyA site of the neo gene, EGFP antisense transcripts were also detectable, however, at a much lower level than the wild-type antisense RNA. (J:106421)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 16 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Carrying any Zfhx2 Mutation:  215 strains or lines available
References
Original:  J:106421 Komine Y, et al., Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci. 2006 Feb;31(2):273-83
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory