Runx1^tm1.1Medv
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1.1Medv
• Name: runt related transcription factor 1; targeted mutation 1.1, Alexander Medvinsky
• MGI ID: MGI:3625886
• Synonyms: Runx1^Re
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1.1Medv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106769
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Expression of a tamoxifen-dependent cre-recombinase from a CAG-TBD-CRE-TBD transgenic construct electroporated into Runx1^tm1Medv ES cells resulted in the excision of the previously inserted floxed sequence (Engrailed2-SA-lacZ), leaving a single loxP site within the 5'UTR of the locus. Northern blot analysis detected normal expression of major splice isoforms in lungs and thymus. (J:106769)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 35 strains or lines available

References

• Original: J:106769 Samokhvalov IM, et al., Multifunctional reversible knockout/reporter system enabling fully functional reconstitution of the AML1/Runx1 locus and rescue of hematopoiesis. Genesis. 2006 Mar;44(3):115-21
• All: 2 reference(s)