About   Help   FAQ
Rb1tm1Fad
Targeted Allele Detail
Nomenclature
Symbol: Rb1tm1Fad
Name: RB transcriptional corepressor 1; targeted mutation 1, Frederick A Dick
MGI ID: MGI:3624796
Synonyms: Rb1deltaL, Rb1deltaLXCXE, Rb1tm1Plam
Gene: Rb1  Location: Chr14:73430298-73563446 bp, - strand  Genetic Position: Chr14, 38.73 cM, cytoband D3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:108366
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Not Specified)
Mutation:    Nucleotide substitutions
 
Mutation detailsA targeting vector was used to insert I740A, N744A, M748A point mutations in the LXCXE binding site. Sequencing of exon 22 confirmed successful recombination. Analysis of mutant fibroblasts confirmed that protein levels were equivalent to wild-type cells. Crossing with mice expressing cre recombinase in the germline removed a floxed neo included in the vector. These substitutions are predicted to make the leucine and cysteine residues of the LXCXE motif a looser fit than the endogenous cleft. (J:108366, J:151417)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rb1 Mutation:  84 strains or lines available
References
Original:  J:108366 Isaac CE, et al., The retinoblastoma protein regulates pericentric heterochromatin. Mol Cell Biol. 2006 May;26(9):3659-71
All:  15 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
05/10/2022
MGI 6.19
The Jackson Laboratory