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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Liqun Luo
MGI ID: MGI:3623347
Synonyms: GR, Gt(ROSA)26Sortm1(EGFP/Dsred2)Luo, Gt(ROSA)26Sortm3Luo, MADM GR knockin
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077333 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:98961
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
Mutation detailsA construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal part of EGFP and C-terminal part of Dsred2 (red fluorescent protein; also tagged with 6 copies of the MYC epitope) separated by a beta-globin intron containing a loxP-flanked neomycin resistance gene, was knocked into ES cells at the ROSA26 locus. No protein is expressed from the chimeric locus in absence of cre-mediated recombination because the coding sequences are interrupted by the intron in different reading frames. After recombination with the reciprocal mutation at the same locus carried by another mouse line, which restores the reading frames of EGFP and RFP, RNA splicing will remove the loxP sites, resulting in functional protein expression. (J:98961)
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  291 strains or lines available
Original:  J:98961 Zong H, et al., Mosaic analysis with double markers in mice. Cell. 2005 May 6;121(3):479-92
All:  8 reference(s)

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MGI 6.01
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