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Spi1tm1.3Dgt
Targeted Allele Detail
Nomenclature
Symbol: Spi1tm1.3Dgt
Name: spleen focus forming virus (SFFV) proviral integration oncogene; targeted mutation 1.3, Daniel G Tenen
MGI ID: MGI:3619530
Synonyms: PU.1 knockdown, UREdelta, UREdelta (neo-)
Gene: Spi1  Location: Chr2:91082390-91115756 bp, + strand  Genetic Position: Chr2, 50.44 cM, cytoband E3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:106040
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Spi1 Mutation:  8 strains or lines available
Notes
Although expression of PU.1/SFPI1 in hematopoietic stem cells, myeloid progenitor cells and granulocytes is similar in mice homozygous for this mutation and for Sfpi1tm1.1Dgt ("UREdeltaneo"), which retains the PGK-neo cassette, mice with the present mutation develop acute myelocytic leukemia (AML) with a lower penetrance (29% vs. 97%) and a longer latency (6-12 months vs. 3-8 months) than do those with Sfpi1tm1.1Dgt. Mice with the present mutation often die of T cell lymphoma before the time of onset of AML. (J:106789;J:90331) The phenotypic difference may be due to genetic background differences, as Sfpi1tm1.1Dgt was analyzed on a ~50% BALB/c and the present mutation on a greater than 50% 129/Sv background.

References
Original:  J:106040 Rosenbauer F, et al., Lymphoid cell growth and transformation are suppressed by a key regulatory element of the gene encoding PU.1. Nat Genet. 2006 Jan;38(1):27-37
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/09/2014
MGI 5.19
The Jackson Laboratory