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Transgene Detail
Symbol: Tg(A94G6)1Rub
Name: transgene insertion 1, Edward M Rubin
MGI ID: MGI:3615509
Transgene: Tg(A94G6)1Rub  Location: unknown  
Strain of Origin:  mixed
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(A94G6)1Rub expresses 8 genes
Mutation detailsThe transgene comprises human YAC clone A94G6, containing a 450 kb segment of human Chr 5q31 that includes, in proximal-to-distal order: SEPT8 (J:40314, not J:60187), KIF3A (J:40314, J:60187), IL4, IL13, RAD50, IL5, IRF1, two putative genes identified by EST matches, and SLC22A5.

RNase protection assays for expression of human SEPT8, KIF3 and RAD50 were performed on 12 tissues from transgenic mice. All three genes showed at least weak expression in every tissue. SEPT8 is strongly expressed in all tissues. KIF3 is expressed strongly in brain, moderately in thymus and weakly in other tissues. RAD50 is expressed strongly in liver and moderately in other tissues. It was noted that expression of KIF3 and RAD50 was detected by RNase protection in some tissues for which no signal was detected by northern blot analysis. (J:40314)

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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Mice homozygous for Del(11Irf1-Csf2)1Rub and hemizygous for the transgene were indistinguishable from wild-type control animals for body weight, liver size, heart size, life span, plasma and hepatic triglyceride levels, and hepatic VLDL triglyceride secretion. As SLC22A5 is the only entire gene present in this transgene, but not in others that failed to complement the homozygous deletion phenotype, the phenotype of Del11Irf1-Csf2)1Rub homozygotes is attributed to loss of this gene.
Original:  J:40314 Frazer KA, et al., Computational and biological analysis of 680 kb of DNA sequence from the human 5q31 cytokine gene cluster region. Genome Res. 1997 May;7(5):495-512
All:  2 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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