Ireb2^tm1Mwh
Targeted Allele Detail

Summary

• Symbol: Ireb2^tm1Mwh
• Name: iron responsive element binding protein 2; targeted mutation 1, Matthias W Hentze
• MGI ID: MGI:3613022
• Synonyms: Irp2^-
• Gene: Ireb2 Location: Chr9:54771073-54819814 bp, + strand Genetic Position: Chr9, 29.81 cM
• Alliance: Ireb2^tm1Mwh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104538
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A gene trap cassette containing an IRES-betageo was introduced into the second intron of the locus. Insertion of the cassette is expected to abrogate the expression of functional protein. The betageo was flanked with frt sites to allow conditional inactivation of the locus and the frame-shifting exon 3 is flanked with loxP sites. Northern blot of mutant tissues detected no change in wild-type transcript levels. Protein was not detected by Western blot or band shift experiments, suggesting that the transcript produced in mutants is abnormal. (J:104538)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ireb2 Mutation: 61 strains or lines available

References

• Original: J:104538 Galy B, et al., Targeted mutagenesis of the murine IRP1 and IRP2 genes reveals context-dependent RNA processing differences in vivo. RNA. 2004 Jul;10(7):1019-25
• All: 4 reference(s)