Pax9^tm1Hpt
Targeted Allele Detail

Summary

• Symbol: Pax9^tm1Hpt
• Name: paired box 9; targeted mutation 1, Heiko Peters
• MGI ID: MGI:3612401
• Synonyms: Pax9^neo
• Gene: Pax9 Location: Chr12:56738552-56759607 bp, + strand Genetic Position: Chr12, 24.53 cM
• Alliance: Pax9^tm1Hpt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104121
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An FRT-flanked neomycin resistance cassette was inserted into intron 2. loxP sites were inserted to flank exons 1 and 2. RT-PCR and sequencing revealed the presence of alternatively spliced forms resulting from the splicing of paired box-containing exon 2 to the 3' region of the Pgk1 promoter. With the neo coding region in frame with exon 2 of Pax9, translation of the mRNA predicts and aberrant fusion protein containing the paired domain and the full-length neomycin phosphotransferase, but lacking most of the C-terminal of Pax9. (J:104121)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax9 Mutation: 16 strains or lines available

References

• Original: J:104121 Kist R, et al., Reduction of Pax9 gene dosage in an allelic series of mouse mutants causes hypodontia and oligodontia. Hum Mol Genet. 2005 Dec 1;14(23):3605-17
• All: 2 reference(s)