Atg5^tm1Nmz
Targeted Allele Detail

Summary

• Symbol: Atg5^tm1Nmz
• Name: autophagy related 5; targeted mutation 1, Noboru Mizushima
• MGI ID: MGI:3612279
• Synonyms: Atg5^-, Atg5^KO
• Gene: Atg5 Location: Chr10:44144354-44240287 bp, + strand Genetic Position: Chr10, 23.24 cM
• Alliance: Atg5^tm1Nmz page

absence of otoconia (right) or the presence of giant and abnormally shaped otoconial crystals (middle) in the utricle of Atg4b^{Gt(A029E06)Wrst}/Atg4b^{Gt(A029E06)Wrst} mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70539
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced exons 2 and 3, including the transcription start site. Protein was not detected in mutant ES cells by immunoblot analysis. (J:104491)

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Atg5^tm1Nmz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atg5 Mutation: 27 strains or lines available

References

• Original: J:70539 Mizushima N, et al., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001 Feb 19;152(4):657-68
• All: 91 reference(s)