About   Help   FAQ
Ep300tm3Pkb
Targeted Allele Detail
Summary
Symbol: Ep300tm3Pkb
Name: E1A binding protein p300; targeted mutation 3, Paul K Brindle
MGI ID: MGI:3612049
Synonyms: p300deltaCH1
Gene: Ep300  Location: Chr15:81470329-81536278 bp, + strand  Genetic Position: Chr15, 38.16 cM
Alliance: Ep300tm3Pkb page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:103607
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted
Mutation:    Intragenic deletion
 
Mutation detailsPortions of the CH1 domain (amino acids 329-379) encoded by exon 4 were deleted and a neomycin resistance cassette flanked by FRT sites was introduced to the downstream intron. Transient infection of the ES cells with a Flp-expressing vector excised the neomycin cassette. RT-PCR confirmed the deletion in mutant MEFs. (J:103607)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Ep300 Mutation:  97 strains or lines available
References
Original:  J:103607 Kasper LH, et al., Two transactivation mechanisms cooperate for the bulk of HIF-1-responsive gene expression. EMBO J. 2005 Nov 16;24(22):3846-58
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory