About   Help   FAQ
Transgene Detail
Symbol: Tg(Lim2*G15V)1Rlc
Name: transgene insertion 1, Robert L Church
MGI ID: MGI:3606782
Synonyms: Lim2To3 transgene
Transgene: Tg(Lim2*G15V)1Rlc  Location: unknown  
Strain of Origin:  (C57BL/6 x SJL)F2
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Lim2*G15V)1Rlc expresses 1 gene
Mutation detailsThe transgene comprises 6.3 kb of genomic DNA containing the entire mouse Lim2 gene, including 399 bp of 5' flanking DNA, with the G-to-T transversion in exon 2 putatively responsible for the dominant total opacity 3 (To3) phenotype. Dot-blot and Southern blot analyses of genomic DNA from transgenic mice indicate presence of two co-integrated copies. RT-PCR of total mouse lens RNA followed by digestion of the PCR product with BstE II, which cuts within the mutant, but not the wildtype, Lim2 cDNA, revealed a low but reproducible ratio of transgenic to wildtype mRNA in lenses from fetal (E18), weanling (P~20) and adult (P>20) transgenic mice. (J:100078)
Inheritance:    Semidominant
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Only one founder, 26011F, transmitted the transgene and the eye phenotype; a second transgenic mouse, also having the eye phenotype, failed to transmit either. Absolute concordance of transgene dosage and the presence and severity of the eye phenotype in >100 progeny of founder 26011F and her offspring verified that the Lim2 mutation identified in To3 mice is responsible for the dominantly inherited To3 phenotype.
Original:  J:100078 Steele EC Jr, et al., Lim2(To3) transgenic mice establish a causative relationship between the mutation identified in the lim2 gene and cataractogenesis in the To3 mouse mutant. Mol Vis. 2000 Jun 2;6:85-94
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.11
The Jackson Laboratory