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Transgene Detail
Symbol: Tg(Lim2*G15V)1Rlc
Name: transgene insertion 1, Robert L Church
MGI ID: MGI:3606782
Synonyms: Lim2To3 transgene
Transgene: Tg(Lim2*G15V)1Rlc  Location: unknown  
Strain of Origin:  (C57BL/6 x SJL)F2
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Lim2*G15V)1Rlc expresses 1 gene
Mutation detailsThe transgene comprises 6.3 kb of genomic DNA containing the entire mouse Lim2 gene, including 399 bp of 5' flanking DNA, with the G-to-T transversion in exon 2 putatively responsible for the dominant total opacity 3 (To3) phenotype. Dot-blot and Southern blot analyses of genomic DNA from transgenic mice indicate presence of two co-integrated copies. RT-PCR of total mouse lens RNA followed by digestion of the PCR product with BstE II, which cuts within the mutant, but not the wildtype, Lim2 cDNA, revealed a low but reproducible ratio of transgenic to wildtype mRNA in lenses from fetal (E18), weanling (P~20) and adult (P>20) transgenic mice. (J:100078)
Inheritance:    Semidominant
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Only one founder, 26011F, transmitted the transgene and the eye phenotype; a second transgenic mouse, also having the eye phenotype, failed to transmit either. Absolute concordance of transgene dosage and the presence and severity of the eye phenotype in >100 progeny of founder 26011F and her offspring verified that the Lim2 mutation identified in To3 mice is responsible for the dominantly inherited To3 phenotype.
Original:  J:100078 Steele EC Jr, et al., Lim2(To3) transgenic mice establish a causative relationship between the mutation identified in the lim2 gene and cataractogenesis in the To3 mouse mutant. Mol Vis. 2000 Jun 2;6:85-94
All:  1 reference(s)

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