Scn3a^{Gt(OST52130)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Scn3a^{Gt(OST52130)Lex}
• Name: sodium channel, voltage-gated, type III, alpha; gene trap OST52130, Lexicon Genetics
• MGI ID: MGI:3604522
• Synonyms: Scn3a^Gt701Lex, Scn3a^Hyp
• Gene: Scn3a Location: Chr2:65288607-65397971 bp, - strand Genetic Position: Chr2, 38.55 cM
• Alliance: Scn3a^{Gt(OST52130)Lex} page

Mutation origin

• Mutant Cell Line: OST52130
• Germline Transmission: Earliest citation of germline transmission: J:103485
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector
• Mutation details: Retroviral insertion occurs just upstream of the first coding exon. PCR analysis on whole brain samples from one-day old (P1) WT, heterozygous and homozygous littermates showed approximately 70% and nearly 100% reduction of mRNA expression from this allele in the brains of heterozygous and homozygous mutants, respectively. Western blotting indicated that protein expression in the brains of P1 heterozygous and homozygous mutants from this allele, compared to WT, was reduced by approximately 35% (p < 0.05) and 60% (p < 0.001), respectively, suggesting that the gene trap mutation is hypomorphic. (J:103485, J:250111)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST52130	CG504283 ; (MGI Seq Detail)	3' RACE	Chr2:65366756-65367034 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Scn3a Mutation: 110 strains or lines available

Notes

See also, data as provided by Lexicon Genetics, Inc.

References

• Original: J:103485 Lexicon Genetics Inc, NIH initiative supporting placement of Lexicon Genetics, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 3 reference(s)