Mchr1^tm1Blak
Targeted Allele Detail

Summary

• Symbol: Mchr1^tm1Blak
• Name: melanin-concentrating hormone receptor 1; targeted mutation 1, Bernard Lakaye
• MGI ID: MGI:3603521
• Synonyms: MCHR1^Neo
• Gene: Mchr1 Location: Chr15:81119700-81123165 bp, + strand Genetic Position: Chr15, 38.02 cM
• Alliance: Mchr1^tm1Blak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101206
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A sequence from exon 2 extending from the third to the seventh transmembrane segment was replaced with a floxed PGK-neo cassette. The receptor-binding pocket was deleted as well as the Asp at amino acid position 123, which is essential for ligand-receptor interaction. Deletion was confirmed by RT-PCR and Northern blot. (J:101206)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mchr1 Mutation: 24 strains or lines available

References

• Original: J:101206 Adamantidis A, et al., Disrupting the melanin-concentrating hormone receptor 1 in mice leads to cognitive deficits and alterations of NMDA receptor function. Eur J Neurosci. 2005 May;21(10):2837-44
• All: 3 reference(s)