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Tg(H2-Ea-Ins2)1Wehi
Transgene Detail
Nomenclature
Symbol: Tg(H2-Ea-Ins2)1Wehi
Name: transgene insertion 1, William and Eliza Hall Institute
MGI ID: MGI:3590216
Transgene: Tg(H2-Ea-Ins2)1Wehi  Location: unknown  
Transgene
origin
Strain of Origin:  NOD/ShiLt
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(H2-Ea-Ins2)1Wehi expresses 1 gene
 
Mutation detailsThe transgene comprises nucleotides -1,903 through -39 of the 5' flanking sequence of the MHC Class II gene H2-Ea (k allele), containing the promoter, followed by a 909 base pair mouse (NOD strain) genomic DNA fragment containing the sequence encoding prepronsulin II and the SV40 small t intron and polyadenylation signal.

Transgene-derived mRNA is detectable in spleen and thymus of transgenic NOD/LtWehi mice, but not of control littermates, by reverse transcription followed by PCR (RT/PCR) using either primers for H2-Eak and proinsulin or two proinsulin primers. Immunoreactive proinsulin is detectable in medium from cultured transgenic splenocytes only after 8 days' culture and at levels 10-2 - 10-3 times those of cultured islets or islet cells; levels are increased by culture with interferon gamma and tumor necrosis factor alpha. (J:100232)

Phenotypes
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Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Two lines of mice were created with this trasgenic construct on the NOD background. No distinction was made between them.

References
Original:  J:100232 French MB, et al., Transgenic expression of mouse proinsulin II prevents diabetes in nonobese diabetic mice. Diabetes. 1997 Jan;46(1):34-9
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/25/2015
MGI 6.0
The Jackson Laboratory