Lhx8^tm1Awan
Targeted Allele Detail

Summary

• Symbol: Lhx8^tm1Awan
• Name: LIM homeobox protein 8; targeted mutation 1, Akio Wanaka
• MGI ID: MGI:3588411
• Gene: Lhx8 Location: Chr3:154011925-154036190 bp, - strand Genetic Position: Chr3, 79.12 cM, cytoband H3-H4
• Alliance: Lhx8^tm1Awan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100340
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A PGK-neomycin resistance gene in reverse orientation replaced exon 7 in order to abolish the core region of the homeodomain. A SIGP cassette was inserted downstream of the neo, thereby inserting a splicing acceptor, IRES, GFP and polyA signal. The expressed transcript would be the fusion of exons 1-6 of Lhx8 and the IRES-GFP-polyA. eGFP expression was not detected by fluorescent microscopy and immunohistochemistry, but was detected by in situ hybridization, suggesting that the translation efficiency of eGFP is very low or it may be suppressed in the mice for an unknon reason. (J:100340)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lhx8 Mutation: 25 strains or lines available

References

• Original: J:100340 Mori T, et al., The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons. Eur J Neurosci. 2004 Jun;19(12):3129-41
• All: 1 reference(s)