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Atg7tm1Tchi
Targeted Allele Detail
Summary
Symbol: Atg7tm1Tchi
Name: autophagy related 7; targeted mutation 1, Tomoki Chiba
MGI ID: MGI:3587769
Synonyms: Atg7F, Atg7Flox, ATG7flox
Gene: Atg7  Location: Chr6:114620075-114837565 bp, + strand  Genetic Position: Chr6, 53.05 cM, cytoband E3
Alliance: Atg7tm1Tchi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:100199
Parent Cell Line:  TT2 (ES Cell)
Strain of Origin:  (C57BL/6NCrlj x CBA/JNCrlj)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA targeting construct was designed to insert a loxP site in intron of the 13th coding exon. Coding exon 14 was fused to a cDNA encoding coding exons 15, 16, and 17 together with a polyadenylation signal after the stop codon. This was then followed by a neomycin resistance cassette and a second loxP site. An additional exon 14 sequence with a stop codon preceding the active site cysteine codon was inserted after the second loxP site. Immunoblot showed that the presence of the loxP sites and neo did not interefere with translation. (J:100199)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 21 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying Atg7tm1Tchi
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Atg7 Mutation:  50 strains or lines available
References
Original:  J:100199 Komatsu M, et al., Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol. 2005 May 9;169(3):425-34
All:  327 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory