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Transgene Detail
Symbol: Tg(Amelx)42Msnd
Name: transgene insertion 42, Malcolm L Snead
MGI ID: MGI:3584497
Synonyms: M180deltaA-FLAG
Transgene: Tg(Amelx)42Msnd  Location: unknown  
Strain of Origin:  (C57BL/6J x DBA/2J)F1 x CBA/J
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Amelx)42Msnd expresses 1 gene
Mutation detailsThe transgene consists of 2.3 kb of genomic DNA containing the amelogenin promoter region and the untranslated first exon, approximately 1.3 kb of intron 1, and the coding sequence for amelogenin - including the signal peptide necessary for secretion of the protein into the extracellular enamel space - with a 27-amino acid sequence containing the FLAG epitope replacing amino acids 3-42. The amelogenin stop codon is followed by the luciferase gene with its polyadenylation signal. (J:100004)
View phenotypes and curated references for all genotypes (concatenated display).
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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
In situ hybridization with a luciferase cDNA probe detects transgene mRNA only in ameloblasts. Immunohistochemical analysis of four day old incisors using antibodies to the FLAG epitope finds tagged amelogenin restricted to ameloblasts, where it is localized to the cytoplasm, and in the secreted organic enamel matrix. (J:100004)

Several independent founder lines generated from this construct were established; no differences were noted among their phenotypes, and no distinction was made among them.

Original:  J:100004 Paine ML, et al., Enamel biomineralization defects result from alterations to amelogenin self-assembly. J Struct Biol. 2000 Dec;132(3):191-200
All:  6 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.12
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