Kras^tm1Bbd
Targeted Allele Detail

Summary

• Symbol: Kras^tm1Bbd
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 1, Mariano Barbacid
• MGI ID: MGI:3582830
• Synonyms: Kras^G12V, Kras^LSL, K-ras^LSLG12Vgeo, Kras^LSLG12Vgeo, K-ras^V12, K^V12
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm1Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86101
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: In one homologous recombination event an IRES Beta-geo cassette was inserted into the 3' untranslated region and in a second homologous recombination event a mutation changing glycine codon 12 in exon 1 into a valine (p.G12V) was inserted along with a loxP site flanked transcriptional stop and hygromycin resistance gene cassette. This resulted in bicistronic Cre recombination-dependent expression of the oncogenic Kras allele and Beta-geo. (J:86101)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm1Bbd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 76 strains or lines available

References

• Original: J:86101 Guerra C, et al., Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context. Cancer Cell. 2003 Aug;4(2):111-20
• All: 53 reference(s)