Id4^tm1Fsky
Targeted Allele Detail

Summary

• Symbol: Id4^tm1Fsky
• Name: inhibitor of DNA binding 4; targeted mutation 1, Fred Sablitzky
• MGI ID: MGI:3579084
• Synonyms: Id4^-, Id4/lacZ
• Gene: Id4 Location: Chr13:48414704-48419502 bp, + strand Genetic Position: Chr13, 24.71 cM, cytoband B
• Alliance: Id4^tm1Fsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98270
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ-neo cassette replaced sequence encoding HLH domain and most of the C-temrinus of the gene. The cassette was inserted in-frame, allowing the expression of a fusion gene encoding the N-terminal 65 amino acids of Id4 fused to beta-galactosidase. RT-PCR of mutant brain and testis failed to detect mRNA encoding the HLH domain. X-gal staining demonstrated that expression of the fusion gene was similar to expression of the endogenous gene expression (J:98270)

Disease models

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Id4 Mutation: 8 strains or lines available

References

• Original: J:98270 Bedford L, et al., Id4 is required for the correct timing of neural differentiation. Dev Biol. 2005 Apr 15;280(2):386-95
• All: 6 reference(s)