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Transgene Detail
Symbol: Tg(Ins2-TFRC/OVA)296Wehi
Name: transgene insertion 296, Walter and Eliza Hall Institute of Medical Research
MGI ID: MGI:3578729
Synonyms: RIP-mOVA
Transgene: Tg(Ins2-TFRC/OVA)296Wehi  Location: unknown  
Strain of Origin:  C57BL/6
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Ins2-TFRC/OVA)296Wehi expresses 2 genes
Mutation detailsThe transgene encodes a fusion protein comprising the carboxy-terminal 118 amino acids of the human transferrin receptor, which include the cytoplasmic tail, membrane-targeting signal and anchor domain of the protein, joined to amino acids 139-385 of mature ovalbumin. Its expression is directed by the rat insulin 2 promoter. (J:98589)
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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Ovalbumin (OV) is present at high levels in pancreatic beta cells and in kidney proximal tubule cells and at very low levels in testes of transgenic mice. No OV has been detected immunohistologically in any of multiple other tissues examined. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an OV-specific T cell receptor expressed on CD8+ T lymphocytes, exhibit thymic deletion of OT-1 T cells, indicating presence of OV at very low levels in the thymus. Western blot analysis reproducibly measured the pancreatic islet OV content as 2.2 ng/ug of protein.

Original:  J:98589 Kurts C, et al., Constitutive class I-restricted exogenous presentation of self antigens in vivo. J Exp Med. 1996 Sep 1;184(3):923-30
All:  62 reference(s)

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MGI 6.01
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