Pou5f1^tm1Scho
Targeted Allele Detail

Summary

• Symbol: Pou5f1^tm1Scho
• Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Hans R Shoeler
• MGI ID: MGI:3578312
• Synonyms: Oct4^flox
• Gene: Pou5f1 Location: Chr17:35816929-35821674 bp, + strand Genetic Position: Chr17, 18.69 cM
• Alliance: Pou5f1^tm1Scho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95317
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: One loxP site was inserted upstream of exon1 and the essential promoter. A floxed neo cassette was inserted into intron 1. The selection cassette was excised by subsequent transient transcription of Cre recombinase in the ES cells. Northern blot analysis demonstrated that a normal sized transcript was expressed from this allele. (J:95317)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou5f1 Mutation: 82 strains or lines available

References

• Original: J:95317 Kehler J, et al., Oct4 is required for primordial germ cell survival. EMBO Rep. 2004 Nov;5(11):1078-83
• All: 12 reference(s)