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Nr3c1tm3Ljm
Targeted Allele Detail
Nomenclature
Symbol: Nr3c1tm3Ljm
Name: nuclear receptor subfamily 3, group C, member 1; targeted mutation 3, Louis J Muglia
MGI ID: MGI:3577992
Synonyms: GFP-GR
Gene: Nr3c1  Location: Chr18:39410545-39519421 bp, - strand  Genetic Position: Chr18, 21.09 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:98328
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed with a GFP fused to the ATG in exon 2 by a 5-amino acid linker, GGSGG. A PGK neo was included in intron 2 and was subsequently removed by crossing with Tg(EIIa-cre)C5379Lmgd mice. Western blot of heterozygotes demonstrated that expression of the GFP-GR fusion protein is equal to that of the endogenous protein. (J:98328)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nr3c1 Mutation:  21 strains or lines available
References
Original:  J:98328 Brewer JA, et al., Green fluorescent protein-glucocorticoid receptor knockin mice reveal dynamic receptor modulation during thymocyte development. J Immunol. 2002 Aug 1;169(3):1309-18
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/11/2020
MGI 6.14
The Jackson Laboratory