Irf3^tm1Ttg
Targeted Allele Detail

Summary

• Symbol: Irf3^tm1Ttg
• Name: interferon regulatory factor 3; targeted mutation 1, Tadatsugu Taniguchi
• MGI ID: MGI:3577601
• Synonyms: IRF-3^-, IRF3^-
• Gene: Irf3 Location: Chr7:44647072-44652272 bp, + strand Genetic Position: Chr7, 29.07 cM, cytoband B2
• Alliance: Irf3^tm1Ttg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98127
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Irf3^tm1Ttg involves 1 genes/genome features (Bcl2l12) View all
• Mutation details: A pgk-neo cassette replaced a 2.2Kb region that contains a putative transcription initiation site and the coding exon for the N-terminal part of the DNA binding domain via homologous recombination. Immunoblot analysis of MEFs confirmed loss of protein. A subsequent publication demonstrated the neo cassette also replaced the first two exons of a second locus located on the opposite strand. This resulted in the loss of the transcription and translational start sites of the second locus. mRNA analysis of numerous tissues from homozygous mice demonstrated transcript levels were 50% to 100% lower than endogenous levels. (J:98127, J:150948)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Irf3 Mutation: 35 strains or lines available

References

• Original: J:98127 Sato M, et al., Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction. Immunity. 2000 Oct;13(4):539-48
• All: 232 reference(s)