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Targeted Allele Detail
Symbol: Irf3tm1Ttg
Name: interferon regulatory factor 3; targeted mutation 1, Tadatsugu Taniguchi
MGI ID: MGI:3577601
Synonyms: IRF-3-, IRF3-
Gene: Irf3  Location: Chr7:44647072-44652272 bp, + strand  Genetic Position: Chr7, 29.07 cM, cytoband B2
Germline Transmission:  Earliest citation of germline transmission: J:98127
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion
  Irf3tm1Ttg involves 1 genes/genome features (Bcl2l12) View all
Mutation detailsA pgk-neo cassette replaced a 2.2Kb region that contains a putative transcription initiation site and the coding exon for the N-terminal part of the DNA binding domain via homologous recombination. Immunoblot analysis of MEFs confirmed loss of protein. A subsequent publication demonstrated the neo cassette also replaced the first two exons of a second locus located on the opposite strand. This resulted in the loss of the transcription and translational start sites of the second locus. mRNA analysis of numerous tissues from homozygous mice demonstrated transcript levels were 50% to 100% lower than endogenous levels. (J:98127, J:150948)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Irf3 Mutation:  32 strains or lines available
Original:  J:98127 Sato M, et al., Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction. Immunity. 2000 Oct;13(4):539-48
All:  218 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.21
The Jackson Laboratory