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Bcl2l12/Irf3tm1Ttg
Targeted Allele Detail
Nomenclature
Symbol: Bcl2l12/Irf3tm1Ttg
Name: interferon regulatory factor 3; targeted mutation 1, Tadatsugu Taniguchi
MGI ID: MGI:3577601
Synonyms: IRF-3-, IRF3-, Irf3tm1Ttg
Gene: Irf3  Location: Chr7:44997648-45002848 bp, + strand  Genetic Position: Chr7, 29.07 cM, cytoband B2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:98127
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsA pgk-neo cassette replaced a 2.2Kb region that contains a putative transcription initiation site and the coding exon for the N-terminal part of the DNA binding domain via homologous recombination. Immunoblot analysis of MEFs confirmed loss of protein. A subsequent publication demonstrated the neo cassette also replaced the first two exons of a second locus located on the opposite strand. This resulted in the loss of the transcription and translational start sites of the second locus. mRNA analysis of numerous tissues from homozygous mice demonstrated transcript levels were 50% to 100% lower than endogenous levels. (J:98127, J:150948)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Irf3 Mutation:  21 strains or lines available
References
Original:  J:98127 Sato M, et al., Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction. Immunity. 2000 Oct;13(4):539-48
All:  115 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/19/2016
MGI 6.04
The Jackson Laboratory