Pfkfb3^tm1Jche
Targeted Allele Detail

Summary

• Symbol: Pfkfb3^tm1Jche
• Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; targeted mutation 1, Jason Chesney
• MGI ID: MGI:3576782
• Synonyms: iPFK2^-
• Gene: Pfkfb3 Location: Chr2:11476241-11558882 bp, - strand Genetic Position: Chr2, 8.73 cM
• Alliance: Pfkfb3^tm1Jche page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97595
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to insert a neomycin cassette and a stop codon to replace exons 3-7, the sequence encoding the substrate binding site and magnesium binding site. RT-PCR of heterozygote lung samples showed a 50% decrease in transcript levels. (J:97595)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfkfb3 Mutation: 36 strains or lines available

References

• Original: J:97595 Chesney J, et al., Targeted disruption of inducible 6-phosphofructo-2-kinase results in embryonic lethality. Biochem Biophys Res Commun. 2005 May 27;331(1):139-46
• All: 3 reference(s)