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Transgene Detail
Symbol: Tg(KRT5-rtTA)1Glk
Name: transgene insertion 1, Adam Glick
MGI ID: MGI:3575756
Synonyms: BK5/rTA, K5/rTA, K5-rtTA
Transgene: Tg(KRT5-rtTA)1Glk  Location: unknown  
Strain of Origin:  FVB/N
Transgene Type:    Transgenic (Transactivator)
Mutation:    Insertion
Mutation detailsThis transgene contains the reverse tetracycline-regulated transactivator gene rtTA under control of the bovine keratin 5 promoter. The rtTA coding sequence lies downstream of approximately 5.2 kilobases of DNA from the 5' flanking region of the keratin followed by a beta-globin intron and upstream of an SV40 polyadenylation signal. (J:65791)
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This is one of 11 lines generated using this transgenic construct. Of the 11, five lines were characterized for their effect on expression of a tetO-lacZ reporter gene in doubly transgenic mice. Such mice, when treated with doxycycline, exhibit 3- or 30-fold elevated levels of beta-galactosidase (b-gal) activity in skin relative to levels in tetO-lacZ singly-transgenic mice. Immunohistochemical staining for b-gal detects expression in skin and hair follicles, specifically in the outer root sheath of hair follicles and, in one line, the basal layer of the epidermis. Lower-level induction occurs in tongue, forestomach and trachea. No significant b-gal is detected in tissues that do not express endogenous keratin 5 or in the absence of doxycycline. (J:65791)
Original:  J:65791 Diamond I, et al., Conditional gene expression in the epidermis of transgenic mice using the tetracycline-regulated transactivators tTA and rTA linked to the keratin 5 promoter. J Invest Dermatol. 2000 Nov;115(5):788-94
All:  23 reference(s)

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MGI 6.07
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