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Tg(KRT5-tTA)1216Glk
Transgene Detail
Summary
Symbol: Tg(KRT5-tTA)1216Glk
Name: transgene insertion 1216, Adam Glick
MGI ID: MGI:3575755
Synonyms: K5-tTA, K5/tTA
Transgene: Tg(KRT5-tTA)1216Glk  Location: unknown  
Alliance: Tg(KRT5-tTA)1216Glk page
Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Transactivator)
Mutation:    Insertion
 
Mutation detailsThis transgene contains the tetracycline-regulated transactivator gene tTA under control of the bovine keratin 5 promoter. The tTA coding sequence lies downstream of approximately 5.2 kilobases of DNA from the 5' flanking region of the keratin gene followed by a beta-globin intron and upstream of an SV40 polyadenylation signal. In the absence of doxycycline, mice doubly transgenic for this transgene and a tetO-lacZ reporter gene exhibit levels of beta-galactosidase(b-gal) activity in skin 500-fold, and in tongue and forestomach 200-300-fold, those of tetO-lacZ singly transgenic mice. Immunohistochemical staining for b-gal detects expression in skin and hair follicles, specifically in the basal layer of the interfollicular epidermis and in the outer root sheath of the hair follicle, and in tongue, forestomach and trachea. No significant b-gal activity is detected in tissues that do not express endogenous keratin 5 or in the presence of doxycycline. (J:65791)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:65791 Diamond I, et al., Conditional gene expression in the epidermis of transgenic mice using the tetracycline-regulated transactivators tTA and rTA linked to the keratin 5 promoter. J Invest Dermatol. 2000 Nov;115(5):788-94
All:  51 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory